منابع مشابه
High-quality frozen extracts of Xenopus laevis eggs reveal size-dependent control of metaphase spindle micromechanics
Cell-free extracts from unfertilized Xenopus laevis eggs offer the opportunity for a variety of biochemical and biophysical assays for analyzing essential cell cycle events such as metaphase spindle assembly. However, the extracts often exhibit substantial variation in quality and have low storage stability, factors that hamper their experimental utility. Here we report a simple two-step method...
متن کاملMetaphase Spindle Assembly
A microtubule-based bipolar spindle is required for error-free chromosome segregation during cell division. In this review I discuss the molecular mechanisms required for the assembly of this dynamic micrometer-scale structure in animal cells.
متن کاملLength Control of the Metaphase Spindle
BACKGROUND The pole-to-pole distance of the metaphase spindle is reasonably constant in a given cell type; in the case of vertebrate female oocytes, this steady-state length can be maintained for substantial lengths of time, during which time microtubules remain highly dynamic. Although a number of molecular perturbations have been shown to influence spindle length, a global understanding of th...
متن کاملInsights into the Micromechanical Properties of the Metaphase Spindle
The microtubule-based metaphase spindle is subjected to forces that act in diverse orientations and over a wide range of timescales. Currently, we cannot explain how this dynamic structure generates and responds to forces while maintaining overall stability, as we have a poor understanding of its micromechanical properties. Here, we combine the use of force-calibrated needles, high-resolution m...
متن کاملA Thermodynamic Analysis of Mitotic Spindle Equilibrium at Active Metaphase
The mitotic apparatus of first-division metaphase eggs of the sea urchin Strongylocentrotus drobachiensis was observed by means of polarization microscopy under controlled temperature conditions. Eggs were fertilized and grown at two temperature extremes in order to produce two different sizes of available spindle pool. Slow division time allowed successive samples of such cells to be observed ...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2015
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.55.255